Novel Small Stable RNAs of Mycoplasma capricolum

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Novel small stable RNAs of Mycoplasma capricolum.

Two stable RNA species and their genes have been isolated from Mycoplasma capricolum, and the nucleotide sequences have been determined by partial RNA sequencing and sequencing of the genes. The RNAs are 92 and 105 nucleotides in length, respectively. The two RNAs reveal no sequence similarity to any stable RNA so far reported, indicating that these are novel RNA species. The RNAs, designated M...

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Stable RNA synthesis and its control in Mycoplasma capricolum.

The synthesis of stable RNA in Mycoplasma capricolum was studied by [32P] labeling of cellular RNA of cells grown in a partially-defined medium in the presence or absence of an amino acid mixture supplement. The results indicate that M. capricolum employs the same stringent control mechanism used by E. coli cells, as judged by a decreased synthesis of stable RNA and accumulation of 5'-triphosph...

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Prevalence of Mycoplasma Capricolum Subspecies Capricolum and Mycoplasma Putrefaciens in Goats in Pishin District of Balochistan

Several Mycoplasma species cause serious and economically important diseases in goats world-wide. Forty goat flocks in the Pishin district of Balochistan province of Pakistan were examined for the clinical cases of contagious caprine pleuropneumonia (CCPP) during 2008. Thirty goats suspected for CCPP on the basis of respiratory symptoms were euthanized for post mortem examination, microbiologic...

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The ribosomal genes of Mycoplasma capricolum.

The nucleotide sequence of 5S rRNA from Mycoplasma capricolum is more similar to that of the gram-positive bacteria than that of the gram-negative bacteria. The presence of two copies of rRNA genes in M. capricolum genome has been demonstrated. The two different rRNA gene clusters have been cloned in E. coli plasmid vectors and analyzed for the rRNA gene organizations, demonstrating that the ge...

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Uptake of fatty acids by Mycoplasma capricolum.

The energy requirements for fatty acid uptake by Mycoplasma capricolum were studied. Fatty acid transport and esterification to phospholipid appeared to be tightly coupled, since there was little intracellular accumulation of free fatty acid. Uptake was blocked by iodoacetate, n-ethylmaleimide, and p-chloromercuribenzoate. Glucose, glycerol, and potassium ions were necessary for fatty acid upta...

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ژورنال

عنوان ژورنال: DNA Research

سال: 1995

ISSN: 1340-2838

DOI: 10.1093/dnares/2.5.229